Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. Generuler 1kb dna ladder, range 250 to 10,000bpcan be labeled radioactively with t4 polynucleotide kinase seeprotocol. Please have a look into the related application note for more information about ladder migration in different gel concentrations. The 1k and 3k bands have increased intensity to serve as reference points. The 1 kb and 3 kb bands contain more dna to provide internal orientation. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. The 1 kb, 3 kb, and 6 kb bands contain more dna to provide internal orientation. Promega 1kb dna ladder molecular weight marker 1kb dna ladder. The approximate mass of dna in each band is provided 0. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis.
We dont know when or if this item will be back in stock. The sizes are 281, 623, 955, 1,383, 1,908, 2,604, 3,638, 4,981 and 6,583 bases. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. The ladder is supplied with 6x dna loading dye, and it can be used in dna sizing and approximate quanitification applications. The pcr markers may be run on polyacrylamide gels with less loading volume. Comparison between 1 kb plus dna ladder and 1 kb dna ladder. View more information about our dna stains technology. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The dna includes fragments ranging from 25010,000 base pairs. Quickload 1 kb extend dna ladder is stable for at least 6 months at 25c. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands.
How can i prepare working solution with 1 kb dna ladder. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. Ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. The doublestranded dna is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated to 10 rnm trishci ph 8. Dna step ladders contain dna bands of defined sizes and equal intensity with fragment lengths at defined increments.
Six bands of equal intensity of 50, 150, 300, 500, 750 and 1,000bp. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. The pcr dna ladder is ideal for routine agarose gel electrophoresis of pcr products or of any dna fragments sized between 100 bp and 2,000 bp. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. The ladder is composed of fifteen chromatographypurified individual dna fragments in base pairs. This marker is ideal for the size determination of digested dna. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The product is supplied in a solution of 10 mm trishcl, ph 8. Thermo scientific generuler 1 kb dna ladder 1kb dna ladder. The 1,000bp and 3,000bp fragments have increased intensity relative to the.
The ladder is dephosphorylated and ready for 5 endlabeling with radioisotopes using t4 polynucleotide kinase, allowing visualization by autoradiography. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. The 1kb dna step ladder has ten bluntended dna fragments ranging from 1kb to 10kb in 1kb increments. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm tris ph 7. This ladder consists of bands containing 18 repeats. The 1 kb dna ladder is suitable for sizing linear doublestranded dna fragments from 250 bp to 10 kb. The ladder is a mixture of chromatographypurified individual dna fragments. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Step ladders are not intended for concentration estimations. For the convenience, the amounts of the 500 and 1,000 bp fragments are adjusted to show greater intensities100 bp dna ladder marker contains 11 double stranded dna fragments from 100bp to 1kb of 100bp increment and 1.
Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. The ladders are not intended for use in quantitative analysis. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Generuler 1 kb plus dna ladder thermo fisher scientific. The ladder is composed of fourteen chromatographypurified. The 500bp fragment is present at increased intensity for easy identification. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder. For example, step ladder dna fragments could be in increments of 100bp from 100bp all the way up to 4000bp. For optimum separation of the highest molecular weight dna fragments, run on a 0. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. Promega dna ladder molecular weight markers, 1kb dna.
Generuler 1 kb dna ladder thermo fisher scientific. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Each ladder is provided with a tube of 6x blueorange loading dye. Generuler 1 kb dna ladder 1250 25 x 50 g for 2500 applications, 0. Description the dna step ladders are ladders of defined sizes with exact incremental steps between bands.
Promega rna markers are suitable for size estimation of singlestranded rna from 0. Dna step ladders have defined sizes with exact incremental steps between bands. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The rna markers consist of a ladder of nine rna transcripts that are synthesized in vitro from specific templates.
For the convenience, the amounts of the 500 and 1,000 bp fragments are adjusted to show greater. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. Promega 1kb dna ladder molecular weight marker 1kb dna. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels. Hyperladder 1kb with 1% wv agarose gel, hyperladder 50bp with 1.
The dna fragments may be stained with ethidium bromide. Dna marker blue bp 1kb dna ladder readytouse dna marker bp 1kb dna ladder is designed for use as molecular weight standard for agarose gel electrophoresis features. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an. The 1,000bp band appears more intense than all other bands, which are of approximately equal intensity. The 200bp dna step ladder has 33 bluntended dna fragments ranging from 200bp to 6,600bp in 200bp increments. All bands except the 5kb band are of equal intensity. Straightforward procedure for laboratory production of dna ladder. Making 1kb ladder working solution university of san diego.
For optimal resolution, the recommended agarose gel concentration is 0. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. Promega dna ladder molecular weight markers, 1kb dna ladder by fisher scientific. This marker can be used with either agarose or polyacrylamide gels.
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